ГОРЕ

проф. д-р Соња Гаџовска-Симиќ

Image descriptionName: Sonja Gadzovska-Simic
Date and place of birth: 21. 8. 1973 in Skopje, Republic of Macedonia
Citizenship/Nationality Macedonian
Marital Status: Married, two children
Office address: Institute of Biology, Faculty of Natural Sciences and Mathematics, Arhimedova 6, Republic of Macedonia
Home Address: Bul. Vidoe Smilevski-Bato 40/16, 1000 Skopje, Republic of Macedonia
Telephone: Office: + 389 2 3249 801, Mob: + 389 78 220 065
Fax: + 389 2 3228 141


Language skills: English, French, Croatian, Serbian


Education – degrees awarded:
1998 Graduated Engineer of Biology (module Biochemistry-Physiology), Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.
1998-2002 Master Studies in Plant Physiology, Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.
Title of Master Thesis:
Hypericin content in in vitro cultures and regenerated plantlets of Hypericum perforatum L. Doctoral studies in Plant Cellular and Molecular Biology, Laboratoire de Biologie des Ligneux et des Grandes Cultures-LBLGC (U.P.R.E.S. E.A. 1207), Faculty of Sciences, University of Orléans, France.
Title of Doctoral Thesis:
Production de métabolites secondaires par les cultures de cellules et de tissus d'Hypericum perforatum L. Effets de divers facteurs exogènes. These de doctorat en Biologie et Physiologie Végétales. Université d'Orléans, France. Secondary metabolite production in cells and tissue cultures of Hypericum perforatum L. Effects of different exogenous factors. (French and English version of the thesis available)


Professional Experience:
1998-2007 Research and Teaching Assistant: Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.
2007- 2013 Assistant Professor: Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.
2007- 2013 Associate Professor: Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril & Methodius” University, Skopje, Republic of Macedonia.


Professional visits & study tours:
2001-2005
• Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, UFR-Faculté de Sciences, Université d’Orléans, France.
• Laboratoire de biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, Université d’Orléans, Chartres, France.
• Centre National de la Recherche Scientifique (CNRS), Centre de Biophysique Moléculaire, Orléans, France.
• Laboratoire de Biologie Moléculaire et Biochimie Végétale, UFR Sciences Pharmaceutiques, Université François-Rabelais, Tours, France.
• Institut National de la Recherche Agronomique (INRA), Centre d’Orléans, France.
2007
• Department of Molecular Biology, Faculty of Science, University of Zagreb, Croatia

Professional membership:
• Association des Doctorants Scientifiques Orléanais (ADSO).
• Association for Medicinal and Aromatic Plants of Southeast European Countries (AMAPSEEC).
• Association Biotechnocentre de la Région Centre, France.
• Croatian Society of Plant Biology(HDBB).
• Serbian Society of Plant Physiology (DFBS).

Teaching experience as Research and Teaching Assistant:
• Plant Physiology (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia)
• Plant Cell and Tissue Culture (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.).


Teaching experience as Assistant Professor:
• Plant Physiology (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia)
• Plant Cell and Tissue Culture (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.).

Teaching experience as Associate Professor:
• Plant Physiology (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia)
• Plant Cell and Tissue Culture (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.).
• Plant Molecular Biology (Institute of Biology, Faculty of Natural Sciences and Mathematics, “Ss. Cyril and Methodius” University, Skopje, Republic of Macedonia.).

Grants and Research Projects:
• (1997-2000) In vitro regeneration of pepper (Capsicum annuum L.) and possibilities for further selection. Ministry of Education and Science, Republic of Macedonia. • (2000-2003) Micropropagation of Hypericum perforatum L. in in vitro conditions and possibilities for production of some secondary metabolites. Ministry of Education and Science, Republic of Macedonia. • (2000-2003) High education of post-graduate net in Biology in south-east Europe (Appui a un reseau d’enseignement superieur post-graduate en biologie en Europe du sud-est). PhD grant supported by the Ministère des Affaires Etrangères (Programme COCOP: Résau d’Enseignement régional Postgraduate en Biologie, grant no. DSUR-NGE-4B1-505).
• (2004-2005) Educational training program for analyses of secondary metabolites in plant cell and tissue cultures, and training of different techniques for analysis of plant metabolites: High performance liquid chromatography (HPLC), Fluorescence Spectroscopy, Mass spectrometry (ESI-MS), in order to implement the acquired expertise in new laboratory activities at home University). Individual Mobility Grant (IMG) financed by TEMPUS program and realized at the University of Orléans, France.
• (2004-2005) Bourse de Formation à la recherche de l’Agence Universitaire de la Francophonie (AUF) à l’Université de Orléans, France.
• (2008-2009) BIVEP – Balkan in vitro Depot of Endangered Plants. Austrian Science and Research Liaison Offices Ljubljana and Sofia. Zentrum für Soziale Innovation. Research Cooperation and Networking between Austria and South Eastern Europe.
• (2009-2010) Application of the research technique in teaching programs Nature, Natural Sciences and Natural Sciences and Techniques. Ministry of Education and Science, Government of the Republic of Macedonia.
• (2008-2011) Centre for Pre-Clinical Testing of Active Substances-CPCTAS. TEMPUS FP7 Program.
• (2011-current project) Procurement of Equipment for Laboratories in the field of Natural and Mathematical sciences: Laboratory for in vitro Plant Cell and Tissue Cultures. Ministry of Education and Science, Government of the Republic of Macedonia (Contract No. 427/2011 dated 12.05.2011).

Scientific experience:
• Plant cell and tissue culture.
• Secondary metabolite production in plant cell and tissue cultures.
• Natural product biosynthesis.
• Influence of biotic and abiotic elicitors on secondary metabolite production.
• HPLC-MS; ESI-MS; GC-MS analyses of natural compounds.
• Activity of key enzymes involved in secondary metabolite production.
• Antioxidant properties of plant extracts.
• Cell redox state.
• Genetic transformation of plant cell by Agrobacterium rhizogenes A4;
• Secondary metabolite production in Genetically Modified Plants;
• Metabolic engineering.

A) List of Publications (2005-2013)
1. Spasenoski, M., Gadzovska, S. (2005): Possibilities for protection of Hypericum perforatum L. with application of in vitro technique. Environmental concern and food safety, 273-276.
2. Spasenoski, M., Gadzovska, S. (2005): Effect of lead (Pb) on the content of chloroplast pigments and accumulation of some mineral elements in poppy (Papaver somniferum L.) during the vegetation. Agric. Sci. Works, 4, 193-202.
3. Gadzovska, S., Maury, S., Ounnar, S., Righezza, M., Kascakova, S., Refregiers, M., Spasenoski, M., Joseph, C., Hagège, D. (2005): Indentification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiology and Biochemistry, 43, 6, 591-601.
4. Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Joseph, C., Hagège, D. (2007): Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tissue and Organ Culture, 89, 1, 1-13.
5. Gadzovska, S., Maury, S., Pavlova, V., Spasenoski, M., Joseph, C., Hagège, D. (2007): Secondary metabolite production in Hypericum perforatum L. in vitro regenerated plantlets. FMNS, 50-54.
6. Pavlova, V., Gadzovska, S., Petrovska-Jovanovic, S. (2007): Simultaneous determination of methylenedioxylated amphetamines in ecstasy tablets by High-performance liquid chromatography. FMNS, 70-74.
7. Gadzovska, S., Nasteska, M., Neskoska, A., Spasenoski, M. (2007): Secondary metabolite productions in different in vitro cultures of marigold (Calendula officinalis L.). Proceedings, 3rd Congr. of Ecologists of Macedonia, 54-61.
8. Gadzovska, S., Dimovska, K., Spasenoski, M. (2007): Phenylpropanoid productions in different in vitro cultures of St. John’s Wort (Hypericum perforatum L.). Proceedings, 3rd Congr. of Ecologists of Macedonia, 47-53.
9. Trpevski, M., Lozanovska, I., Talevska, A., Ugurovska, D., Spasenoski, M., Gadzovska, S. (2007): Phenolic and flavonoid contents of some medicinal plants from Jablanica Mt., Republic of Macedonia. Proceedings, 3rd Congr. of Ecologists of Macedonia, 40-46.
10. Gadzovska-Simic, S., Maury, S., Pavlova, V., Delaunay, A., Spasenoski, M., Joseph, C., Hagège, D. (2008): Phenylpropanoid productions in Hypericum perforatum L. in vitro cultures. Proceedings, XX Congr. of Chem. and Tehchnol. of Macedonia, BFT-06-E, 1-4.
11. Gadzovska-Simic, S., Savreska, H., Pavlova, V., Tusevski, O., Brndevska, N., Spasenoski, M. (2008): Effects of salicylic acid on phenylpropanoid productions in Calendula officinalis L. in vitro shoots. Proceedings, XX Congr. of Chem. and Tehchnol. of Macedonia, BFT-07-E, 1-4.
12. Gadzovska-Simic, S., Pavlova, V., Tusevski, O., Brndevska, N., Spasenoski, M. (2008): Effects of fungal elicitor Aspergillus flavus on phenylpropanoid productions in Hypericum perforatum L. cell suspensions. Proceedings, XX Congr. of Chem. and Tehchnol. of Macedonia, BFT-08-E, 1-4.
13. Pavlova, V., Gadzovska-Simic, S., Petrovska-Jovanovic, S. (2008): Development and optimization of SPE method for determination of some methylenedioxy amphetamines in urine. Proceedings, XX Congr. of Chem. and Tehchnol. of Macedonia, ACE-07-E, 1-3.
14. Pavlova, V., Gadzovska-Simic, S., Petrovska-Jovanovic, S. (2008): Optimization of derivatization procedure for analysis of primary, secondary and tertiary amines. Proceedings, XX Congr. of Chem. and Tehchnol. of Macedonia, ACE-08-E, 1-4.
15. Tusevski, O., Trpevski, M., Lozanovska, I., Talevska, A., Ugurovska, D., Gadzovska- Simic, S. (2010): Antioxidant activity of phenolic compounds in methanolic extracts of some Macedonian medicinal plants collected on Jablanica Mountain. Bull. Biol. Stud. Res. Soc. 4, 103-111.
16. Gadzovska-Simic, S., Tusevski, O., Antevski, S., Atanasova-Pancevska, N., Petreska, J., Stefova, M., Kungulovski, Dz., Spasenoski, M. (2012): Secondary metabolite production in Hypericum perforatum L. cell suspensions upon elicitation with fungal mycelia from Aspergillus flavus. Archive of Biological Sciences, 64, 1, 113-121.
17. Tusevski O., Petreska Stanoeva J., Stefova M., Kungulovski Dz., Atanasova Pancevska N., Panov S., Gadzovska-Simic S. (2012): Xanthone production in Hypericum perforatum hairy root cultures transformed with Agrobacterium rhizogenes A4. Proceedings, Conference on Medicinal and Aromatic plants of South East European Countries, 7th CMAPSEEC, 381-388.
18. Tusevski, O., Gadzovska Simic, S. (2013): Phenolic acids and flavonoids in Hypericum perforatum L. hairy roots. International Journal of Pharma and Bio Sciences, 4, (3), 737-748.
19. Tusevski, O., Petreska Stanoeva, J., Stefova, M., Kungulovski, Dz., Atanasova Pancevska, N., Sekulovski, N., Panov, S., Gadzovska Simic, S. (2013): Hairy roots of Hypericum perforatum L.: a promising system for xanthone production. Central European Journal of Biology, 8, (10), 1010-1022.
20. Tusevski, O., Petreska Stanoeva, J., Stefova, M., Gadzovska Simic, S. (2013): Phenolic profile of dark-grown and photoperiod-exposed Hypericum perforatum L. hairy root cultures. The Scientific World Journal, (In Press).Tusevski, О., Kostovska, А., Iloska, А., Trajkovska, Lj., Gadzovska Simic, S. Phenolic production and antioxidant properties of some Macedonian medicinal plants. Central European Journal of Biology, (accepted for publication).

Books:
1. Spasenoski, M., Gadzovska-Simic, S. (2009): Fiziologija na rastenijata, Plant Physiology, Eds., Andreeva, L., Tomovski, Z., Urumov, V., Karadelev, M., Vasilevski, D., Bakeva, V., Risteski, Lj. Prirodno-matematicki fakultet, Skopje, ISBN 978-9989-668-79-1, pp. 449.
2. Gadzovska-Simic, S. (2010): Secondary metabolite production in cultures in vitro of Hypericum perforatum L. Effects of different exogenous factors. LAP Lambert Academic Publishing, Saarbrücken, Germany, ISBN 978-3-8383-8304-0, pp.1-192. (Book is available on English language).
3. Gadzovska-Simic, S., Spasenoski, M., Tusevski, O. (2011): Микропропагација на растенијата: култивирање на клетки и ткива во in vitro услови. Plant Micropropagation: cultivation of cells and tissues in in vitro conditions. (“Ss. Cyril & Methodius” University Bulletin No. 1016, 03.10.2011). (Book is available on Macedonian language).

B) Participation in scientific conference and congress (2005-2013)
1. Spasenoski, M., Gadzovska, S. (2005): Possibilities for protection of Hypericum perforatum L. with application of in vitro technique. 1st Congress of Plant Protection, 28. XI- 2. XII 2005, Ohrid, Macedonia.
2. Spasenoski, M., Gadzovska, S. (2005): Effect of lead (Pb) on the content of chloroplast pigments and accumulation of some mineral elements in poppy (Papaver somniferum L.) during the vegetation. Agricultural University - Plovdiv, 60th Jubilee scientific conference “State-of-the-art and problems of agricultural science and education”, 18-20 October 2005, Plovdiv, Bulgaria.
3. Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Joseph, C., Hagège, D. (2006): Jasmonic acid elicitation of phenylpropanoids and naphtodianthrones in Hypericum perforatum L. cell suspensions. IV Balkan Botanical Congress, 20-26 June 2006, Sofia, Bulgaria.
4. Gadzovska, S. (2007): Effects of different elicitors on secondary metabolite productions in Hypericum perforatum L. in vitro cultures, Croatian Society of Plant Physiologists, Faculty of Sciences, 30 May 2007, Zagreb, Croatia.
5. Gadzovska, S., Maury, S., Pavlova, V., Spasenoski, M., Joseph, C., Hagège, D. (2007): Secondary metabolite production in Hypericum perforatum L. in vitro regenerated plantlets. International Scientific Conference FMNS-2007, 6-10 June 2007, Blagoevgrad, Bulgaria.
6. Pavlova, V., Gadzovska, S., Petrovska-Jovanovic, S. (2007): Simultaneous determination of methylenedioxylated amphetamines in ecstasy tablets by High-performance liquid chromatography. International Scientific Conference FMNS-2007, 6-10 June 2007, Blagoevgrad, Bulgaria.
7. Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Joseph, C., Hagège, D. (2007): Production of phenylpropanoids and naphtodianthrones in Hypericum perforatum L. in vitro regenerated plantlets. International Scientific Conference Propagation of Ornamental Plants, 5-8 September 2007, Sofia, Bulgaria.
8. Markoska, E., Petkovska, A., Spasenoski, M., Stefova, M., Gadzovska, S. (2007): Secondary metabolite production in Astragalus parnassi Boiss. plant. 4th Congress of Pharmacy of Macedonia with International Participation, 26-30 September 2007, Ohrid, Macedonia.
9. Gadzovska, S., Nasteska, M., Neskoska, A., Spasenoski, M. (2007): Secondary metabolite productions in different in vitro cultures of marigold (Calendula officinalis L.). 3rd Congress of Ecologists of the Republic of Macedonia, 6-9 October 2007, Struga, Macedonia.
10. Gadzovska, S., Dimovska, K., Spasenoski, M. (2007): Phenylpropanoid productions in different in vitro cultures of St. John’s Wort (Hypericum perforatum L.). 3rd Congress of Ecologists of the Republic of Macedonia, 6-9 October 2007, Struga, Macedonia.
11. Trpevski, M., Lozanovska, I., Talevska, A., Ugurovska, D., Spasenoski, M., Gadzovska, S. (2007): Phenolic and flavonoid contents of some medicinal plants from Jablanica Mt., Republic of Macedonia. 3rd Congress of Ecologists of the Republic of Macedonia, 6-9 October 2007, Struga, Macedonia.
12. Gadzovska-Simic, S., Maury, S., Pavlova, V., Delaunay, A., Spasenoski, M., Joseph, C., Hagège, D. (2008): Phenylpropanoid productions in Hypericum perforatum L. in vitro cultures. XX Congress of Chemists and Tehchnologists of Macedonia, 17-20 September, Ohrid, Macedonia.
13. Gadzovska-Simic, S., Pavlova, V., Tusevski, O., Brndevska, N., Spasenoski, M. (2008): Effects of fungal elicitor Aspergillus flavus on phenylpropanoid productions in Hypericum perforatum L. cell suspensions. XX Congress of Chemists and Tehchnologists of Macedonia, 17-20 September, Ohrid, Macedonia.
14. Gadzovska-Simic, S., Savreska, H., Pavlova, V., Tusevski, O., Brndevska, N., Spasenoski, M. (2007): Effects of salicylic acid on phenylpropanoid productions in Calendula officinalis L. in vitro shoots. XX Congress of Chemists and Tehchnologists of Macedonia, 17-20 September, Ohrid, Macedonia.
15. Pavlova, V., Gadzovska-Simic, S., Petrovska-Jovanovic, S. (2008): Development and optimization of SPE method for determination of some methylenedioxy amphetamines in urine. XX Congress of Chemists and Tehchnologists of Macedonia, 17-20 September, Ohrid, Macedonia.
16. Pavlova, V., Gadzovska-Simic, S., Petrovska-Jovanovic, S. (2008): Optimization of derivatization procedure for analysis of primary, secondary and tertiary amines. XX Congress of Chemists and Tehchnologists of Macedonia, 17-20 September, Ohrid, Macedonia.
17. Gadzovska-Simic, S. (2009): BIVEP - Balkan in vitro culture depot of endangered plant species, ASO - BIVEP Workshop at the Institute of Botany – Bulgarian Academy of Sciences with participants from Austria, Serbia, Bosnia-Herzegovina, Croatia, Macedonia, and Bulgaria, 19-21 February, Sofia, Bulgaria.
18. Gadzovska-Simic, S. (2009): Antioxidant activity of secondary metabolites produced by different Hypericum perforatum L. in vitro cultures. Workshop for regional networking of researchers in the field of antioxidants characterization. Institute of Chemistry, Faculty of Natural Sciences and Mathematics, 12-15 November, Skopje, Macedonia.
19. Gadzovska-Simic, S., Tusevski, O., Kungulovski, Dz., Atanasova-Pancevska, N., Stefova, M. (2011): Phenylpropanoid production in Hypericum perforatum L. cells upon co-cultivation with Agrobacterium rhizogenes A4. Preclinical testing of active substances and cancer research, Internationally recognized symposium on Anticancer agents, Cardiotoxicity and Neurotoxicity, 16-19 March, Kragujevac, Serbia.
20. Gadzovska-Simic, S., Tusevski, O., Stefova, M., Kungulovski, Dz., Atanasova-Pancevska, N., (2011): Hypericin and pseudohypericin production in elicited Hypericum perforatum L. cell provides antioxidant protection. Preclinical testing of active substances and cancer research, Internationally recognized symposium on Anticancer agents, Cardiotoxicity and Neurotoxicity, 16-19 March, Kragujevac, Serbia.
21. Gadzovska-Simic, S. (2011): Effect of biotic elicitors on hypericin and pseudohypericin production in Hypericum perforatum L. cell suspensions. 19th DFBS Symposium of Serbian Society for Plant Physiology, 13-15 June, Vrujci, Serbia.
22. Gadzovska-Simic, S., Tusevski O., Stefova M., Petreska, J. (2011): Secondary metabolite profiles in different in vitro cultures of Hypericum perforatum L. 19th DFBS Symposium of Serbian Society for Plant Physiology, 13-15 June, Vrujci, Serbia.
23. Tusevski, O., Gadzovska-Simic, S., Pavlova, V. (2011): Determination of flavonoids in Varumin® oral solutions. Euroanalysis 16, 16th European Conference of Analytical Chemistry, 11-15 September, Belgrade, Serbia.
24. Tusevski O., Petreska Stanoeva J., Stefova M., Kungulovski Dz., Atanasova Pancevska N., Panov S., Gadzovska-Simic S. (2012): Xanthone production in Hypericum perforatum hairy root cultures transformed with Agrobacterium rhizogenes A4. 7th Conference on Medicinal and Aromatic plants of South East European Countries, 7th CMAPSEEC, 27-30 May, Subotica, Serbia.
25. Hristovski, S., Gadzovska Simic, S., Melovski, Lj., Tusevski, O., Kotevski, I. (2012): Dynamics of soluble phenolic compounds in the course of litter decomposition in the beech ecosystem Calamintho grandiflorae-Fagetum in Mavrovo National Park, Republic of Macedonia. IV Congress of the Ecologists of Macedonia, 12-15 October 2012, Ohrid, Macedonia. Abstract book, pp. 25-26.
26. Nikolovska Nedelkoska, D., Tusevski, O., Rusevska, K., Gadzovska Simic, S., Karadelev, M. (2012): Antioxidant capacity of methanolic extracts of selected bolets mushrooms collected on Galichica Mountain, Macedonia. IV Congress of the Ecologists of Macedonia, 12-15 October 2012, Ohrid, Macedonia. Abstract book, pp. 46-47.
27. Dodevska, I., Kostovska, A. Petruseva, D., Mitkovska, M., Tusevski, O., Gadzovska Simic, S. (2012): The cupric ion reducing antioxidant capacity and phenolic content in methanolic extracts of some Macedonian medicinal plants collected on Galichitsa Mountain. IV Congress of the Ecologists of Macedonia, 12-15 October 2012, Ohrid, Macedonia. Abstract book, pp. 182.
28. Tusevski, O., Kostovska, A., Iloska, A., Gadzovska Simic, S. (2013): Antioxidant activity of Hypericum perforatum L. hairy root extracts. 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, 4-7 June 2013, Subotica, Serbia. Abstract book, pp. 99-100.
29. Gadzovska Simic, S., Tusevski, O., Petreska Stanoeva, J., Stefova, M. (2013): Phenolic acids and flavonoids idetified in Hypericum perforatum L. hairy roots. 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, 4-7 June 2013, Subotica, Serbia. Abstract book, pp. 100.